Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
Plasmid: pAS2
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 8500
- Notes
- Restriction digests of the clone give the following sizes (kb): BamHI--8.6; SalI--8.6; SmaI--8.6. (ATCC staff) Shuttle expression vector used to create fusion proteins consisting of the nuclear localization sequence from SV40 T antigen, the GAL4 DNA-binding domain (aa 1-147), and a HA (hemagglutinin) epitope tag in frame with the activation domain. [1] The order of the major features in this plasmid is: ADC1 (ADH) promoter -> - GAL4 DNA binding domain - HA - NdeI/MCS/SalI - ADC1 terminator - pMB1 ori - ampR - 2 micron ori - TRP1 -> - f1 ori - <- CYH2. [2] Growth: LB plus ampicillin (ATCC medium number 1227) 37C Deposited by: Elledge S.J. Hosts: E.coli, yeast, Saccharomyces cerevisiae. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number
- 87008
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified