Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
Plasmid: pFL26
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 5558
- Notes
- pFL26 was constructed from pUC19 plasmid where two alu I sites were modified.The site 629 was replaced by a BglII linker and the site 747 by a ClaI site. The yeast selectable marker has been cloned in the BglII site. The order of the major features in this plasmid is:EcoRI/MCS/HindIII - LEU2 - pMB1 ori - ampR. YI-type shuttle integration vector permitting visual detection of recombinants based on alpha complementation. Restriction digests of the clone give the following sizes (kb): BamHI--5.6; BglII--2.8 (doublet); ClaI--3.6, 1.9. (ATCC staff) Medium is 1227 LB plus ampicillin. NCBI gi: 397127 Hosts: E.coli HB101, S.cerevisiae, E.coli, Saccharomyces. Related vectors: pUC19, yeast 2-micron plasmid. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number
- 77199
- GenBank
- X70477
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified