Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: pSLH8
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 4007
- Notes
- A high-copy-number cloning vector permitting visual detection of recombinants using insertional inactivation of the Rhodococcus gene for indigo production. Includes priming sites useful for sequencing. [1] The indigo pigment is intracellular and is produced on unsupplemented LB agar. Production is subject to catabolite repression by glucose, but is enhanced by tryptophan, indole, lactose or IPTG. [1] The M13 reverse sequencing primer and a 17mer primer [(5'-3') (CGAAGGCCGGTACCAGG)] complementary to the 5' end of the pigment gene (nt 849-832) can be used for sequencing cloned DNA inserts. [1] The portion of the indigo-producing gene retained in the construct corresponds to nt 791-2109 of the reported sequence. [1] The order of the major features in this plasmid is: lacOP - EcoRI/MCS/SphI - indigo - lacZ' remnant - ampR - pMB1 ori - lacI. [1] Restriction digests of the clone give the following sizes (kb): EcoRI/XbaI--2.7, 1.4; EcoRI--4.1; BamHI--4.1. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: E.coli LK111, E.coli. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number
- 77285
- GenBank
- M55641, M30195
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified