Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: pSupexp
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 5500
- Notes
- Deposited by: T. Subramanian Shuttle expression vector permitting levels of protein production in human cells high enough for immunoprecipitation. [1] Contains two mammalian transcription units: the foreign DNA regulated by HIV-1 LTR and an SV40 polyadenylation sequence, and HIV-1 tat and neo regulated by an SV40 early promoter and polyadenylation sequence. [1] The AflII and SpeI cloning sites are recommended for the 5' end of foreign DNA to prevent potential translation effects of ATG sequences in the multiple cloning site. [1] The order of the major features in this plasmid is: AatII - HIV-1 LTR - MCS - SV40 polyadenylation - SV40 early promoter - tat - neoR - SV40 polyadenylation - pMB1 ori - ampR. [1] Restriction digests of the clone give the following sizes (kb): HindIII--5.5; NotI--5.5; KpnI--5.5. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: E.coli, E.coli HB101. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number
- 77362
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified