Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: pTacNeo1.2
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 5440
- Notes
- The upstream translation of LacZ is out of frame with APH(3')-I. (personal communication) The vector contains pBR322 (EcoRI-SphI), a TAC promoter, the first 8 amino-terminal codons of beta-galactosidase, & a gene encoding a modified aminoglycoside phosphotransferase (3')-I (APH(3')-I). (personal communication) The start of the modified APH(3')-I is followed by the eleventh codon (serine) of APH(3')-I. Codons 2-10 have been deleted. (personal communication) Sequences from the TAA stop codon of APH(3')-I to the immediate downstream PvuII site encode a portion of Tn601. Sequences from the PvuII site to the PstI site are derived from Tn5. (personal communication) This is an expression vector capable of producing a beta-galactosidase: insert: APH(3')-I fusion protein. (personal communication) Restriction digests of the clone give the following sizes (kb): BamHI--5.45; XhoI--5.45; EcoRI--5.45; PvuI--4.0, 1.45; PstI--3.1, 2.35. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: E.coli DG101, vertebrate cells, E.coli. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number
- 37687
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified