Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: pTYB2
Information
- Source/Vendor
- New England Biolabs
- Plasmid Type
- Bacterial Expression
- Cloning Method
- Unknown
- Size
- 7474
- 5' Sequencing 1 Primer
- T7
- Tag 1
- Intein
- Bacterial Resistance
- Ampicillin
- Notes
- pTYB2 is an E. coli cloning and expression vector (7474 bp) used in the IMPACT Protein Purification System which allows the overexpression of a target protein as a fusion to a self-cleavable affinity tag (1,2). This C-terminal fusion vector is designed for the in-frame insertion of a target gene into the polylinker upstream of an intein tag (the Sce VMA intein/chitin binding domain, 55 kDa). This results in the fusion of the C-terminus of the target protein to the N-terminus of the intein tag. Thiol-induced self-cleavage of the intein releases the target protein from the chitin-bound intein tag, resulting in a single column purification of the target protein.
- Catalog Number
- N6702S
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified