Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
Plasmid: YRp15-leu2delta1
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 7700
- Notes
- A marker addition/transplacement vector. [1] A 0.6 kb EcoRI/ClaI fragment (blunt-ended) was deleted from a 2.2 kb genomic SalI/XhoI fragment having a SalI linker. The SalI fragment was cloned into YRp15. [1] Restriction digests of the clone give the following sizes (kb): EcoRI/ClaI--6.1, 1.6; SalI--5.9, 1.8; EcoRI/HindIII--6.2, 1.5; HindIII--7.7. (ATCC staff) Non-integrants grow poorly selecting for Ura3+ (pinpoints at 5 days) while integrants grow well because the SUP11 copy number is maintained at one. If there are no integrants on a transformation plate, streak onto -URA plates and pick papillae. (personal communication) Medium is 1227 LB plus ampicillin. Hosts: E.coli HB101, Saccharomyces cerevisiae, E.coli. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number
- 77147
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified